What happens if a centrifuge is unbalanced?

What happens if a centrifuge is unbalanced?

Unbalanced centrifuge rotors can result in injury or death. Sample container breakage can release aerosols that are harmful if inhaled. The majority of all centrifuge accidents result from user error.

Why does a centrifuge separate blood?

Centrifugal force is used to separate the components of blood – red blood cells, platelets and plasma – from each other. The result is that the particles with different densities precipitate in layers.

How platelets cells are separated?

During a platelet donation, whole blood is drawn from one arm into a sterile kit inside a cell separating machine. The machine separates the blood so that only platelets and plasma are collected. The other blood components (red cells and white cells) are returned to the donor via the same arm.

Why does blood separate in a tube?

Blood sample tubes that contain a separating gel are often used to obtain blood serum for laboratory tests. After centrifugation, the inert acrylic gel at the bottom of the tube normally occupies the middle position between the cells (clot) and the serum, as its density is intermediate between theirs.

What happens if you spin blood before it clots?

If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This finding is frequent in hemolyzed specimens. Also, the gel barrier may not be intact and could cause improper separation of serum and cells, possibly affecting test results.

How long can a CBC sit at room temperature?

24 hours

What is the correct order of draw?

The recommended order is as follows: Blood culture tubes. Sodium citrate tubes (e.g., blue-stopper) Serum tubes with or without clot activator, with or without gel separator (e.g., red-, gold-, speckled-stopper)

Do you spin down lavender tubes?

—To obtain plasma, the anticoagulated specimen may be spun down within minutes of draw. Transfer of serum or plasma into an appropriately labeled tube must be done within 1 hour after centrifugation. After centrifugation, the gel should be intact and cells and serum completely separated.

How many times do you invert a GREY tube?

NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.

How many times can you invert tubes?

All tubes (except red top tubes which contain no additives) must be gently inverted 5 to 8 times immediately after filling, to ensure proper mixing of blood and anticoagulant, or other additives.

What color tube is used for sodium level test?


Red No additive 7.0 mL
Light Blue 3.2% Sodium Citrate 4.5 mL
Gold Top (Serum Separator, “SST”) Contains separating gel and clot activator 6.0 mL
Light Blue – Yellow Label on Tube Thrombin 2.0 mL

What is the correct order of blood draw tubes?

The draw order for specimen tubes is as follows: Gold SST (Plain tube w/gel and clot activator additive) Green and Dark Green (Heparin, with and without gel) Lavender (EDTA) Pink – Blood Bank (EDTA)

What color tube is used for hematology?

Lavender top tube – EDTA EDTA

What test goes in what color tube?

Blood Collection Tubes

Tube cap color Additive Common laboratory tests
Lavender or pink Potassium EDTA Hematology and blood bank
Gray Sodium fluoride, and sodium or potassium oxalate Glucose (especially when testing will be delayed), blood alcohol, lactic acid

What color tube is used for syphilis?


What color tube is CRP?

ORDERING INFORMATION: Geisinger Epic Procedure Code: LAB2100 Geisinger Epic ID: 21624
Specimen type: Plasma or serum
Preferred collection container: Stat/Line draws: 3 mL green/yellow-top (plasma separator) tube Routine requests/off-site specimens: 3.5 mL gold-top (serum separator) tube

What color tube is used for BMP test?

Red-top tube or green-top (lithium heparin) tube is acceptable. Gel-barrier tube (send entire tube) is preferred. Red-top tube or green-top ( heparin) tube is acceptable. Gel-barrier tube (send entire tube) is preferred.

What color tube is used for sedimentation rate?


Does a BMP need to be spun?

BASIC METABOLIC PANEL Allow serum to clot. Centrifuge to separate serum or plasma. Specimen is not acceptable for glucose or potassium determination if not centrifuged within two hours of collection time.

What does BMP check for?

A basic metabolic panel is a blood test that measures your sugar (glucose) level, electrolyte and fluid balance, and kidney function. Glucose is a type of sugar your body uses for energy. Electrolytes keep your body’s fluids in balance.

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