What is enzyme specific activity?

What is enzyme specific activity?

Specific enzyme activity (usually stated simply as ‘specific activity’) is the number of enzyme units per ml divided by the concentration of protein in mg/ml. Specific activity values are therefore quoted as units/mg or nmol/min/mg (if unit definition B is applied).

What are examples of enzymes?

Examples of specific enzymes

  • Lipases – a group of enzymes that help digest fats in the gut.
  • Amylase – helps change starches into sugars.
  • Maltase – also found in saliva; breaks the sugar maltose into glucose.
  • Trypsin – found in the small intestine, breaks proteins down into amino acids.

What pH is best for enzyme activity?


Does pH affect rate of reaction?

Optimal pH increases enzyme rate of reaction while less than optimal pH decreases it. Increasing temperature also increases enzyme rate of reaction, until things get too hot, then the enzyme denatures and ceases to function.

Does pH affect km?

Originally Answered: Can pH affect Michaelis constant? Yes – the Michaelis constant is only constant for a constant temperature and pH. Changing either of those will affect an enzyme’s ability to catalyse a reaction.

What is a pH of 10?

Pure water has a neutral pH of 7. pH values lower than 7 are acidic, and pH values higher than 7 are alkaline (basic)….What does it mean for a solution to be acidic or basic (alkaline)?

pH Value H+ Concentration Relative to Pure Water Example
10 0.001 Great Salt Lake, milk of magnesia

What does pH measure?

pH is a measure of how acidic/basic water is. pHs of less than 7 indicate acidity, whereas a pH of greater than 7 indicates a base. pH is really a measure of the relative amount of free hydrogen and hydroxyl ions in the water.

Why P in pH is small?

pH is an old abbreviation for a french description of the acidity of water. The French term is “puissance d’hydrogen”, which means “power or strength of Hydrogen”. The p is small because it refers to a word.

What are 3 characteristics of acids?


  • Aqueous solutions of acids are electrolytes, meaning that they conduct an electrical current.
  • Acids have a sour taste.
  • Acids change the color of certain acid-base indicators.
  • Acids react with active metals to yield hydrogen gas.

How is pH calculated?

To calculate the pH of an aqueous solution you need to know the concentration of the hydronium ion in moles per liter (molarity). The pH is then calculated using the expression: pH = – log [H3O+]. Example: What is the pOH of a solution that has a hydroxide ion concentration of 4.82 x 10-5 M?

What is the pH of HCl?


What is the pH of 10-2 M HCl?

Solution : pH of 10-2 M NaOH solution = 12. pH of 10-2 M HCl = 2.

What is the pH of pure water?

The pH of pure water (H20) is 7 at 25oC, but when exposed to the carbon dioxide in the atmosphere this equilibrium results in a pH of approximately 5.2.

Is pH 10 Water Safe?

Drinking natural alkaline water is generally considered safe, since it contains natural minerals. However, you should use caution with artificial alkaline water, which likely contains fewer good minerals than its high pH would have you believe, and may contain contaminants.

Why is pH of water 7?

Even in pure water ions tend to form due to random processes (producing some H+ and OH- ions). The amount of H+ that is made in pure water is about equal to a pH of 7. That’s why 7 is neutral. In pure water near room temperature, the concentration of H+ is about 10-7 moles/liter, which gives a pH of 7.

Is pH 9 Water Safe?

Some people say that drinking slightly alkaline water — with a pH between 8 and 9 — can improve your health. They say it may make you age more slowly, maintain a healthy pH in your body, and block chronic disease like cancer.

What pH is dangerous to skin?

The issue is based on tap water having a pH of 7 and being perceived as too high for skin’s pH of 5.5 to 4.5.

Is 9.5 pH water good?

Healthline reveals that “normal drinking water generally has a neutral pH of 7; alkaline water typically has a pH of 8 or 9.” Results show that alkaline water is more beneficial by being able to effectively neutralize the acid in your body compared to other waters.

What pH level is harmful to humans?

Although the pH of blood ranges from 7.35-7.45, the pH of other body fluids is different. pH indicates the level of H+ ions, where low pH indicates too many H+ ions and high pH indicates too many OH- ions. If the pH levels drop below 6.9, it can lead to coma.

What pH is safe to drink?

The U.S. Environmental Protection Agency recommends that the pH level of water sources should be at a pH measurement level between 6.5 to 8.5 on a scale that ranges from 0 to 14. The best pH of drinking water sits right in the middle at a 7.

What pH are humans?

Life on earth depends on appropriate pH levels in and around living organisms and cells. Human life requires a tightly controlled pH level in the serum of about 7.4 (a slightly alkaline range of 7.35 to 7.45) to survive [1].

What is the pH of urine?

Normal urine pH is slightly acidic, with usual values of 6.0 to 7.5, but the normal range is 4.5 to 8.0.

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